kemalandClaude Opus 5.5
0215af9c9c
CPIC lookups by allele function counts or activity values (duplications N x activity, *5/*0 = 0, total-score fallback), Indeterminate when feasible solutions disagree or an allele is not in the guideline; per-sample simurg.pgx/1 JSON and diplotype_annotations.tsv with PGX-026 metadata lines. Co-Authored-By: Claude Opus 5.5 <[email protected]> Claude-Session: https://claude.ai/code/session_01814GKVzZVL3FuF3DeiZXhv
1303 lines
63 KiB
Python
1303 lines
63 KiB
Python
"""``simurg`` command-line interface (spec §2, plan §14).
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Available: ``version``, ``inspect`` (P1), ``ingest`` (P2), ``normalize`` (P3), ``genotype`` (P4).
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Later phases add the other commands of CLI-001.
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"""
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from __future__ import annotations
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import json
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import os
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import sys
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from collections.abc import Callable
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from pathlib import Path
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from typing import Annotated, Any, TypeVar
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import numpy as np
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import typer
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from simurg import PRODUCT_NAME, __version__
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from simurg.core.errors import ExitCode, SimurgError
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from simurg.core.log import Reporter
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from simurg.core.provenance import git_revision
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app = typer.Typer(
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name="simurg",
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help=f"{PRODUCT_NAME}: analysis toolkit for Illumina Infinium genotyping BeadChip data.",
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no_args_is_help=True,
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add_completion=False,
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pretty_exceptions_enable=False,
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context_settings={"help_option_names": ["-h", "--help"]},
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)
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F = TypeVar("F", bound=Callable[..., Any])
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def version_string() -> str:
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rev = git_revision()
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return f"{PRODUCT_NAME} {__version__}" + (f"+{rev}" if rev else "")
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def _version_cb(value: bool) -> None:
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if value:
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typer.echo(version_string())
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raise typer.Exit(0)
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@app.callback()
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def main(
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version: Annotated[
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bool, typer.Option("--version", callback=_version_cb, is_eager=True, help="Print the version and exit.")
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] = False,
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) -> None:
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"""Top-level options."""
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def run_guarded(reporter: Reporter, fn: Callable[[], int]) -> None:
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"""Run a command body mapping errors to catalogue codes and exit codes (CLI-014)."""
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reporter.open()
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code = ExitCode.OK
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try:
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code = ExitCode(fn())
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sys.stdout.flush() # surface a closed pipe here, not at interpreter exit
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except BrokenPipeError:
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# the consumer of stdout stopped reading (`| head`): silence further writes, exit like a SIGPIPE'd tool
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os.dup2(os.open(os.devnull, os.O_WRONLY), sys.stdout.fileno())
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code = ExitCode.SIGPIPE
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except SimurgError as e:
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reporter.error(e.code, e.message + (f"\n hint: {e.hint}" if e.hint else ""), exc=e, file=e.path)
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code = e.exit_code
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except KeyboardInterrupt:
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reporter.error("E_INTERNAL", "interrupted")
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code = ExitCode.SIGINT
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except Exception as e:
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reporter.error(
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"E_INTERNAL", f"internal error: {type(e).__name__}: {e}. Rerun with --debug and report it.", exc=e
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)
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code = ExitCode.INTERNAL
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finally:
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reporter.close()
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raise typer.Exit(int(code))
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# --------------------------------------------------------------------------------------------------------
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@app.command("version")
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def version_cmd() -> None:
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"""Print the version."""
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typer.echo(version_string())
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# --------------------------------------------------------------------------------------------------------
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def _jsonable(o: Any) -> Any:
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if isinstance(o, np.generic):
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return o.item()
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if isinstance(o, np.ndarray):
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return o.tolist()
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if isinstance(o, Path):
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return str(o)
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if isinstance(o, bytes):
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return o.hex()
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if isinstance(o, float) and o != o: # NaN
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return None
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return str(o)
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def inspect_file(path: Path) -> dict[str, Any]:
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from simurg.formats.detect import detect_kind
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kind = detect_kind(path)
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info: dict[str, Any] = {"file": str(path), "kind": kind}
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if kind == "idat":
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from simurg.formats.idat import read_idat
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md = read_idat(path).metadata()
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md.pop("path", None)
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info.update(md)
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elif kind == "bpm":
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from simurg.formats.bpm import read_bpm
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info.update(read_bpm(path).summary())
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elif kind == "csv_manifest":
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from simurg.formats.csv_manifest import assay_format, read_csv_manifest
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m = read_csv_manifest(path, load_sequences=False)
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s = m.summary()
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s.pop("normalization_bins", None)
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s.pop("infinium_i_at", None)
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s.pop("infinium_i_gc", None)
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s["heading"] = m.heading
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s["assay_format"] = assay_format(m)
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info.update(s)
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elif kind == "egt":
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from simurg.formats.egt import read_egt
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info.update(read_egt(path).summary())
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elif kind == "gtc":
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from simurg.formats.gtc import read_gtc, validate_gtc
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g = read_gtc(path, check_write_complete=False)
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info.update(g.summary())
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info["problems"] = validate_gtc(g)
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elif kind == "samplesheet":
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from simurg.formats.samplesheet import parse_samplesheet
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sh = parse_samplesheet(path)
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info.update(
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{
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"rows": len(sh.rows),
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"columns": sh.columns,
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"key_columns": [sh.barcode_column, sh.position_column],
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"root_folder": str(sh.root_folder) if sh.root_folder else None,
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"header": sh.header,
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"has_sample_name": sh.has_sample_name,
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"warnings": [f"{c}: {m}" for c, m in sh.warnings],
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}
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)
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else:
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raise SimurgError("E_FILE_UNREADABLE", f"{path}: unrecognised file type", path=str(path))
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info.pop("path", None)
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return info
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def _print_human(info: dict[str, Any]) -> None:
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typer.echo(f"== {info['file']} ({info['kind']})")
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for k, v in info.items():
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if k in ("file", "kind"):
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continue
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if isinstance(v, list) and len(v) > 12:
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v = [*v[:12], f"… ({len(v)} total)"]
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if isinstance(v, (dict, list)):
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v = json.dumps(v, default=_jsonable, ensure_ascii=False)
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typer.echo(f" {k}: {v}")
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@app.command("inspect")
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def inspect_cmd(
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files: Annotated[list[Path], typer.Argument(help="IDAT, BPM, CSV manifest, EGT, GTC or sample-sheet files.")],
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as_json: Annotated[bool, typer.Option("--json", help="Machine-readable JSON output.")] = False,
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log_json: Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")] = False,
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debug: Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")] = False,
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log_level: Annotated[str, typer.Option("--log-level", help="debug|info|warning|error")] = "info",
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quiet: Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")] = False,
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) -> None:
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"""Print the header / table of contents of array files (spec CLI-020)."""
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rep = Reporter(
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command="inspect", level="debug" if debug else log_level, json_stderr=log_json, debug=debug, quiet=quiet
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)
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def body() -> int:
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results = []
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failures = 0
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for f in files:
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if not f.exists():
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raise SimurgError("E_FILE_NOT_FOUND", f"{f}: no such file", path=str(f))
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try:
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info = inspect_file(f)
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except SimurgError as e:
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rep.error(e.code, e.message, file=str(f))
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failures += 1
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continue
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results.append(info)
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if not as_json:
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_print_human(info)
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if as_json:
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out = results[0] if len(results) == 1 else results
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sys.stdout.write(json.dumps(out, default=_jsonable, ensure_ascii=False, indent=2) + "\n")
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if failures and not results:
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return ExitCode.USAGE
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return ExitCode.PARTIAL if failures else ExitCode.OK
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run_guarded(rep, body)
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# --------------------------------------------------------------------------------------------------------
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@app.command("ingest")
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def ingest_cmd(
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bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
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out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
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idats: Annotated[Path | None, typer.Option("--idats", help="Folder searched recursively for IDAT pairs.")] = None,
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sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
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csv: Annotated[Path | None, typer.Option("--csv", help="CSV manifest, checked against the BPM.")] = None,
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threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
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force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
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resume: Annotated[bool, typer.Option("--resume", help="Keep outputs whose inputs are unchanged.")] = False,
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layout: Annotated[str, typer.Option("--layout", help="nested|flat")] = "nested",
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on_duplicate_idat: Annotated[str, typer.Option("--on-duplicate-idat", help="error|first|newest")] = "error",
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log_json: Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")] = False,
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debug: Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")] = False,
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log_level: Annotated[str, typer.Option("--log-level", help="debug|info|warning|error")] = "info",
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quiet: Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")] = False,
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) -> None:
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"""IDAT pairs + BPM → canonical per-sample raw intensities (Parquet). Phase P2."""
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from simurg.pipeline.ingest import IngestOptions, run_ingest
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if layout not in ("nested", "flat"):
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raise typer.BadParameter("--layout must be nested or flat")
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if on_duplicate_idat not in ("error", "first", "newest"):
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raise typer.BadParameter("--on-duplicate-idat must be error, first or newest")
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rep = Reporter(
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command="ingest",
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level="debug" if debug else log_level,
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json_stderr=log_json,
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debug=debug,
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quiet=quiet,
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log_file=out / "logs" / "ingest.log",
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)
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opts = IngestOptions(
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out=out,
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bpm=bpm,
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idats=idats,
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sheet=sheet,
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csv=csv,
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threads=threads,
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force=force,
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resume=resume,
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layout=layout,
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on_duplicate_idat=on_duplicate_idat,
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argv=sys.argv[1:],
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)
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run_guarded(rep, lambda: run_ingest(opts, rep))
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# --------------------------------------------------------------------------------------------------------
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@app.command("normalize")
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def normalize_cmd(
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indir: Annotated[Path, typer.Option("--in", help="Output folder of `simurg ingest`.")],
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norm_version: Annotated[str, typer.Option("--norm-version", help="1.2.0 (current) or 1.1.2 (older)")] = "1.2.0",
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threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
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force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
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log_json: Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")] = False,
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debug: Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")] = False,
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quiet: Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")] = False,
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) -> None:
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"""Fit per-bin normalization transforms and write normalized X/Y, theta and R (phase P3)."""
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from simurg.pipeline.normalize import NormalizeOptions, run_normalize
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rep = Reporter(
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command="normalize", level="debug" if debug else "info", json_stderr=log_json, debug=debug, quiet=quiet
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)
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opts = NormalizeOptions(indir=indir, version=norm_version, threads=threads, force=force, argv=sys.argv[1:])
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run_guarded(rep, lambda: run_normalize(opts, rep))
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# --------------------------------------------------------------------------------------------------------
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@app.command("genotype")
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def genotype_cmd(
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bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
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egt: Annotated[Path, typer.Option("--egt", help="EGT cluster file (required).")],
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out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
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idats: Annotated[Path | None, typer.Option("--idats", help="Folder searched recursively for IDAT pairs.")] = None,
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sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
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csv: Annotated[Path | None, typer.Option("--csv", help="CSV manifest, checked against the BPM.")] = None,
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cutoff: Annotated[float, typer.Option("--cutoff", help="GenCall score below which a call is a no-call.")] = 0.15,
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preset: Annotated[
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str, typer.Option("--preset", help="current (default) or autocall-1.6.2 (legacy vendor behaviour)")
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] = "current",
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norm_version: Annotated[
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str | None, typer.Option("--norm-version", help="1.2.0 or 1.1.2 (default: from --preset)")
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] = None,
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sex_version: Annotated[
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int | None, typer.Option("--sex-version", min=1, max=2, help="Sex estimate rule 1 or 2 (default: preset)")
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] = None,
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gtc_version: Annotated[
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int | None, typer.Option("--gtc-version", help="GTC format 3 or 5 (default: preset)")
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] = None,
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autocall_date: Annotated[
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str | None, typer.Option("--autocall-date", help="Fixed value for GTC field 201 (reproducible output).")
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] = None,
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threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
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controls: Annotated[
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bool, typer.Option("--controls/--no-controls", help="Write controls_raw.tsv and controls_qc.tsv.")
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] = True,
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cohort_qc: Annotated[
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bool, typer.Option("--cohort-qc/--no-cohort-qc", help="Contamination and relatedness after genotyping.")
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] = True,
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qc_config: Annotated[Path | None, typer.Option("--qc-config", help="QC threshold YAML (QC-020).")] = None,
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qc_profile: Annotated[str | None, typer.Option("--qc-profile", help="QC profile name or 'auto'.")] = None,
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threshold_call_rate: Annotated[float | None, typer.Option("--threshold-call-rate")] = None,
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threshold_lrr_sd: Annotated[float | None, typer.Option("--threshold-lrr-sd")] = None,
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threshold: Annotated[list[str] | None, typer.Option("--threshold", help="KEY=VALUE (repeatable).")] = None,
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bg_offset: Annotated[float | None, typer.Option("--bg-offset", help="Control background offset (3000).")] = None,
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force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
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layout: Annotated[str, typer.Option("--layout", help="nested|flat")] = "nested",
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on_duplicate_idat: Annotated[str, typer.Option("--on-duplicate-idat", help="error|first|newest")] = "error",
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log_json: Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")] = False,
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debug: Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")] = False,
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quiet: Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")] = False,
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) -> None:
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"""IDAT pairs → GTC files and a sample summary (GenCall-equivalent calling; phase P4)."""
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from simurg.pipeline.genotype import GenotypeOptions, run_genotype
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if layout not in ("nested", "flat"):
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raise typer.BadParameter("--layout must be nested or flat")
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from simurg.genotype.sample import PRESETS
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if preset not in PRESETS:
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raise typer.BadParameter(f"--preset must be one of {sorted(PRESETS)}")
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if gtc_version not in (None, 3, 5):
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raise typer.BadParameter("--gtc-version must be 3 or 5")
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if norm_version not in (None, "1.2.0", "1.1.2"):
|
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raise typer.BadParameter("--norm-version must be 1.2.0 or 1.1.2")
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rep = Reporter(
|
||
command="genotype",
|
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level="debug" if debug else "info",
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json_stderr=log_json,
|
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debug=debug,
|
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quiet=quiet,
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log_file=out / "logs" / "genotype.log",
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)
|
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opts = GenotypeOptions(
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out=out,
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bpm=bpm,
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egt=egt,
|
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idats=idats,
|
||
sheet=sheet,
|
||
csv=csv,
|
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threads=threads,
|
||
force=force,
|
||
layout=layout,
|
||
on_duplicate_idat=on_duplicate_idat,
|
||
cutoff=cutoff,
|
||
preset=preset,
|
||
norm_version=norm_version,
|
||
sex_version=sex_version,
|
||
gtc_version=gtc_version,
|
||
autocall_date=autocall_date,
|
||
controls=controls,
|
||
cohort_qc=cohort_qc,
|
||
qc_config=qc_config,
|
||
qc_profile=qc_profile,
|
||
threshold_call_rate=threshold_call_rate,
|
||
threshold_lrr_sd=threshold_lrr_sd,
|
||
thresholds=list(threshold or []),
|
||
bg_offset=bg_offset,
|
||
argv=sys.argv[1:],
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||
)
|
||
run_guarded(rep, lambda: run_genotype(opts, rep))
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
@app.command("gtc-to-vcf")
|
||
def gtc_to_vcf_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
fasta: Annotated[Path, typer.Option("--fasta", help="Reference FASTA (plain or bgzip; required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
csv: Annotated[Path | None, typer.Option("--csv", help="CSV manifest (SourceSeq; required for indels).")] = None,
|
||
no_bgzip: Annotated[bool, typer.Option("--no-bgzip", help="Plain VCF, no index.")] = False,
|
||
genome_cache: Annotated[bool, typer.Option("--genome-cache/--no-genome-cache", help="mmap genome cache.")] = True,
|
||
exclude_loci: Annotated[
|
||
Path | None, typer.Option("--exclude-loci", "--filter-loci", help="Probe names to drop (one per line).")
|
||
] = None,
|
||
include_loci: Annotated[Path | None, typer.Option("--include-loci", help="Only these probe names.")] = None,
|
||
unsquash_duplicates: Annotated[bool, typer.Option("--unsquash-duplicates", help="One record per probe.")] = False,
|
||
inf1_nocall_informative: Annotated[
|
||
bool, typer.Option("--inf1-nocall-informative", help="Read Infinium I no-calls as allele absence.")
|
||
] = False,
|
||
drop_ambiguous: Annotated[bool, typer.Option("--drop-ambiguous", help="Drop low-confidence strand loci.")] = False,
|
||
filter_gs: Annotated[float | None, typer.Option("--filter-gs", help="FILTER LowGS when GS < X.")] = None,
|
||
contig_alias: Annotated[str, typer.Option("--contig-alias", help="strict|auto|FILE")] = "strict",
|
||
ploidy_policy: Annotated[str, typer.Option("--ploidy-policy", help="sex-aware|diploid")] = "sex-aware",
|
||
threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
|
||
merge: Annotated[
|
||
bool, typer.Option("--merge", help="One multi-sample BCF (+ .csi) instead of per-sample VCFs.")
|
||
] = False,
|
||
auxiliary_loci: Annotated[
|
||
Path | None, typer.Option("--auxiliary-loci", help="VCF of true MNV definitions for manifest probes (OUT-035).")
|
||
] = None,
|
||
merge_snv_indel: Annotated[
|
||
bool, typer.Option("--merge-snv-indel", help="One record for an SNV and an indel at the same position.")
|
||
] = False,
|
||
info_fields: Annotated[
|
||
str,
|
||
typer.Option(
|
||
"--info-fields", help="Comma list: ASSAY_TYPE,NORM_BIN,GENTRAIN,CLUSTER_SEP,ILMN_STRAND,PROBE_IDS"
|
||
),
|
||
] = "",
|
||
extra_format: Annotated[
|
||
str, typer.Option("--extra-format", help="Comma list: X,Y,NORMX,NORMY,THETA,R (single-probe records)")
|
||
] = "",
|
||
egt: Annotated[Path | None, typer.Option("--egt", help="Cluster file (INFO GENTRAIN, CLUSTER_SEP).")] = None,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")] = False,
|
||
) -> None:
|
||
"""GTC files → one bgzipped, indexed SNV VCF per sample (plus-strand alleles, merged multi-probe sites)."""
|
||
from simurg.pipeline.vcf import VcfOptions, run_gtc_to_vcf
|
||
|
||
rep = Reporter(
|
||
command="gtc-to-vcf",
|
||
level="debug" if debug else "info",
|
||
json_stderr=log_json,
|
||
debug=debug,
|
||
quiet=quiet,
|
||
log_file=out / "logs" / "gtc-to-vcf.log",
|
||
)
|
||
opts = VcfOptions(
|
||
out=out, bpm=bpm, fasta=fasta, gtcs=gtcs, sheet=sheet, csv=csv, bgzip=not no_bgzip, genome_cache=genome_cache,
|
||
exclude_loci=exclude_loci, include_loci=include_loci, unsquash_duplicates=unsquash_duplicates,
|
||
inf1_nocall_informative=inf1_nocall_informative, drop_ambiguous=drop_ambiguous, filter_gs=filter_gs,
|
||
contig_alias=contig_alias, ploidy_policy=ploidy_policy, threads=threads, merge=merge,
|
||
merge_snv_indel=merge_snv_indel,
|
||
auxiliary_loci=auxiliary_loci,
|
||
force=force,
|
||
info_fields=[x.strip().upper() for x in info_fields.split(",") if x.strip()],
|
||
extra_format=[x.strip().upper() for x in extra_format.split(",") if x.strip()], egt=egt,
|
||
argv=sys.argv[1:],
|
||
) # fmt: skip
|
||
run_guarded(rep, lambda: run_gtc_to_vcf(opts, rep))
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
@app.command("compare")
|
||
def compare_cmd(
|
||
a: Annotated[Path, typer.Argument(help="GTC/VCF file or folder (A).")],
|
||
b: Annotated[Path, typer.Argument(help="GTC/VCF file or folder (B), paired with A by relative path.")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (summary.json, discrepancies, dashboard).")],
|
||
bpm: Annotated[Path | None, typer.Option("--bpm", help="Manifest, for per-locus-class counts of GTCs.")] = None,
|
||
tolerance: Annotated[
|
||
list[str] | None,
|
||
typer.Option("--tolerance", help="FIELD=exact|abs=X|ulp=N (repeatable); VCF floats: float=abs=X"),
|
||
] = None,
|
||
sample: Annotated[
|
||
list[str] | None, typer.Option("--sample", help="VCF sample(s) to compare (default: all shared).")
|
||
] = None,
|
||
title: Annotated[str, typer.Option("--title", help="Dashboard title.")] = "",
|
||
threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
|
||
fail_on_discrepancy: Annotated[
|
||
bool, typer.Option("--fail-on-discrepancy", help="Exit 3 when any DISCREPANCY is found.")
|
||
] = False,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress")] = False,
|
||
) -> None:
|
||
"""Field-level differential test of two GTCs/VCFs (or folders): EXACT/NEAR/DISCREPANCY/UNKNOWN (plan §16.3)."""
|
||
from simurg.pipeline.compare import CompareOptions, run_compare
|
||
|
||
rep = Reporter(
|
||
command="compare", level="debug" if debug else "info", json_stderr=log_json, debug=debug, quiet=quiet
|
||
)
|
||
|
||
def body() -> int:
|
||
tol: dict[str, str] = {}
|
||
for item in tolerance or []:
|
||
key, sep, val = item.partition("=")
|
||
if not sep:
|
||
raise SimurgError("E_CONFIG_KEY", f"--tolerance expects FIELD=VALUE, got {item!r}")
|
||
tol[key.strip()] = val.strip()
|
||
opts = CompareOptions(
|
||
a=a,
|
||
b=b,
|
||
out=out,
|
||
bpm=bpm,
|
||
tolerances=tol,
|
||
samples=list(sample) if sample else None,
|
||
threads=threads,
|
||
fail_on_discrepancy=fail_on_discrepancy,
|
||
title=title,
|
||
force=force,
|
||
)
|
||
return run_compare(opts, rep)
|
||
|
||
run_guarded(rep, body)
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
export_app = typer.Typer(help="Exports from GTCs or VCFs: bedgraph, finalreport, plink, locus-summary.")
|
||
app.add_typer(export_app, name="export")
|
||
|
||
|
||
def _plain_reporter(command: str, log_json: bool, debug: bool, quiet: bool) -> Reporter:
|
||
return Reporter(command=command, level="debug" if debug else "info", json_stderr=log_json, debug=debug, quiet=quiet)
|
||
|
||
|
||
@export_app.command("bedgraph")
|
||
def export_bedgraph_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
no_gzip: Annotated[bool, typer.Option("--no-gzip", help="Plain .bedgraph files.")] = False,
|
||
chrom_style: Annotated[str, typer.Option("--chrom-style", help="manifest|ucsc")] = "manifest",
|
||
smoothed_lrr: Annotated[int, typer.Option("--smoothed-lrr", min=0, help="Extra moving-median LRR track, K.")] = 0,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress")] = False,
|
||
) -> None:
|
||
"""BAF and LRR bedGraph tracks per sample (OUT-050 … OUT-052)."""
|
||
from simurg.pipeline.export import BedgraphOptions, run_export_bedgraph
|
||
|
||
rep = _plain_reporter("export bedgraph", log_json, debug, quiet)
|
||
opts = BedgraphOptions(out=out, bpm=bpm, gtcs=gtcs, sheet=sheet, gzip=not no_gzip, chrom_style=chrom_style,
|
||
smoothed_lrr=smoothed_lrr, force=force, argv=sys.argv[1:]) # fmt: skip
|
||
run_guarded(rep, lambda: run_export_bedgraph(opts, rep))
|
||
|
||
|
||
@export_app.command("finalreport")
|
||
def export_finalreport_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
csv: Annotated[Path | None, typer.Option("--csv", help="CSV manifest (source/reference strands).")] = None,
|
||
egt: Annotated[Path | None, typer.Option("--egt", help="Cluster file (GT Score column).")] = None,
|
||
matrix: Annotated[bool, typer.Option("--matrix", help="Loci × samples genotype matrix.")] = False,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress")] = False,
|
||
) -> None:
|
||
"""Long-format final report (one row per locus × sample), or a genotype matrix (OUT-071)."""
|
||
from simurg.pipeline.export import FinalReportOptions, run_export_finalreport
|
||
|
||
rep = _plain_reporter("export finalreport", log_json, debug, quiet)
|
||
opts = FinalReportOptions(out=out, bpm=bpm, gtcs=gtcs, sheet=sheet, csv=csv, egt=egt, matrix=matrix, force=force,
|
||
argv=sys.argv[1:]) # fmt: skip
|
||
run_guarded(rep, lambda: run_export_finalreport(opts, rep))
|
||
|
||
|
||
@export_app.command("plink")
|
||
def export_plink_cmd(
|
||
vcfs: Annotated[Path, typer.Option("--vcfs", help="Folder with SimurgArray per-sample *.snv.vcf.gz.")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (reported sex).")] = None,
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="GTC folder (estimated sex).")] = None,
|
||
multiallelic: Annotated[str, typer.Option("--multiallelic", help="split|drop")] = "split",
|
||
fmt: Annotated[str, typer.Option("--format", help="bed (PLINK 1), pgen (PLINK 2) or both")] = "bed",
|
||
prefix: Annotated[str, typer.Option("--prefix", help="Output file prefix.")] = "cohort",
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress")] = False,
|
||
) -> None:
|
||
"""PLINK 1 .bed/.bim/.fam from SimurgArray VCFs (OUT-043)."""
|
||
from simurg.pipeline.export import PlinkOptions, run_export_plink
|
||
|
||
rep = _plain_reporter("export plink", log_json, debug, quiet)
|
||
opts = PlinkOptions(
|
||
out=out,
|
||
vcfs=vcfs,
|
||
sheet=sheet,
|
||
gtcs=gtcs,
|
||
multiallelic=multiallelic,
|
||
format=fmt,
|
||
prefix=prefix,
|
||
force=force,
|
||
argv=sys.argv[1:],
|
||
)
|
||
run_guarded(rep, lambda: run_export_plink(opts, rep))
|
||
|
||
|
||
@export_app.command("locus-summary")
|
||
def export_locus_summary_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
egt: Annotated[Path, typer.Option("--egt", help="EGT cluster file (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
threads: Annotated[int, typer.Option("--threads", min=0, max=1024)] = 0,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: Annotated[bool, typer.Option("--log-json", "--json-log")] = False,
|
||
debug: Annotated[bool, typer.Option("--debug")] = False,
|
||
quiet: Annotated[bool, typer.Option("--quiet", "--no-progress")] = False,
|
||
) -> None:
|
||
"""Per-locus summary statistics (OUT-072); same as ``simurg qc loci``."""
|
||
_qc_cohort("loci", out, bpm, gtcs, sheet, egt, threads, force, log_json, debug, quiet)
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
qc_app = typer.Typer(help="Quality control: controls, samples, contamination, relatedness, loci, thresholds.")
|
||
app.add_typer(qc_app, name="qc")
|
||
|
||
|
||
def _reporter(command: str, out: Path | None, log_json: bool, debug: bool, quiet: bool) -> Reporter:
|
||
return Reporter(
|
||
command=command,
|
||
level="debug" if debug else "info",
|
||
json_stderr=log_json,
|
||
debug=debug,
|
||
quiet=quiet,
|
||
log_file=(out / "logs" / f"{command.replace(' ', '_')}.log") if out is not None else None,
|
||
)
|
||
|
||
|
||
LogJson = Annotated[bool, typer.Option("--log-json", "--json-log", help="JSON Lines log on stderr.")]
|
||
Debug = Annotated[bool, typer.Option("--debug", help="Debug logging with tracebacks.")]
|
||
Quiet = Annotated[bool, typer.Option("--quiet", "--no-progress", help="Suppress info messages.")]
|
||
|
||
|
||
@qc_app.command("controls")
|
||
def qc_controls_cmd(
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
array_type: Annotated[str, typer.Option("--array-type", help="genotyping|methylation (required).")],
|
||
bpm: Annotated[Path | None, typer.Option("--bpm", help="BPM manifest (control definitions).")] = None,
|
||
csv: Annotated[Path | None, typer.Option("--csv", help="CSV manifest (control definitions).")] = None,
|
||
idats: Annotated[Path | None, typer.Option("--idats", help="Folder searched recursively for IDAT pairs.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
bg_offset: Annotated[float, typer.Option("--bg-offset", help="Background offset x (QC-003).")] = 3000.0,
|
||
compat: Annotated[bool, typer.Option("--compat-column-names", help="Reference-style CSV names.")] = False,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing control tables.")] = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Control-probe QC from IDATs without genotyping (QC-012)."""
|
||
from simurg.pipeline.qc import QcControlsOptions, run_qc_controls
|
||
|
||
if array_type not in ("genotyping", "methylation"):
|
||
raise typer.BadParameter("--array-type must be genotyping or methylation")
|
||
rep = _reporter("qc controls", out, log_json, debug, quiet)
|
||
opts = QcControlsOptions(
|
||
out=out,
|
||
bpm=bpm,
|
||
csv=csv,
|
||
idats=idats,
|
||
sheet=sheet,
|
||
array_type=array_type,
|
||
bg_offset=bg_offset,
|
||
compat=compat,
|
||
force=force,
|
||
argv=sys.argv[1:],
|
||
)
|
||
run_guarded(rep, lambda: run_qc_controls(opts, rep))
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
cn_app = typer.Typer(help="Genome-wide copy number, LOH and mosaicism (CYTO-001).")
|
||
app.add_typer(cn_app, name="cn")
|
||
|
||
|
||
@cn_app.command("build-model")
|
||
def cn_build_model_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
fasta: Annotated[Path, typer.Option("--fasta", help="Reference FASTA of the manifest's build (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output model file (.simurg-cyto).")],
|
||
egt: Annotated[Path | None, typer.Option("--egt", help="Cluster file: population B-allele frequencies.")] = None,
|
||
reference_gtcs: Annotated[
|
||
Path | None, typer.Option("--reference-gtcs", help="GTCs of normal samples (>= 24) for the batch reference.")
|
||
] = None,
|
||
max_reference: Annotated[int, typer.Option("--max-reference", min=1, help="Random subset size.")] = 100,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Cyto model: GC covariates, PFB and an optional batch reference per probe (CYTO-038, IN-051)."""
|
||
from simurg.pipeline.cn import run_build_model
|
||
|
||
rep = _reporter("cn build-model", out.parent, log_json, debug, quiet)
|
||
run_guarded(rep, lambda: run_build_model(bpm, fasta, out, rep, egt, "auto", reference_gtcs, max_reference))
|
||
|
||
|
||
def _kb(v: int) -> int:
|
||
if not 0 <= v <= 250_000:
|
||
raise typer.BadParameter("sizes are 0-250000 kb")
|
||
return v * 1000
|
||
|
||
|
||
@cn_app.command("call")
|
||
def cn_call_cmd(
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
model: Annotated[Path, typer.Option("--model", help="Cyto model from cn build-model (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
gtcs: Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")] = None,
|
||
summary: Annotated[
|
||
Path | None, typer.Option("--summary", help="sample_summary.tsv (default: next to GTCs).")
|
||
] = None,
|
||
fasta: Annotated[Path | None, typer.Option("--fasta", help="FASTA for REF padding bases.")] = None,
|
||
min_del_probes: Annotated[int, typer.Option("--min-del-probes", min=0, max=250000)] = 10,
|
||
min_del_size: Annotated[int, typer.Option("--min-del-size", help="kb")] = 25,
|
||
min_dup_probes: Annotated[int, typer.Option("--min-dup-probes", min=0, max=250000)] = 10,
|
||
min_dup_size: Annotated[int, typer.Option("--min-dup-size", help="kb")] = 50,
|
||
min_loh_probes: Annotated[int, typer.Option("--min-loh-probes", min=0, max=250000)] = 500,
|
||
min_loh_size: Annotated[int, typer.Option("--min-loh-size", help="kb")] = 3000,
|
||
min_gainloh_probes: Annotated[int, typer.Option("--min-gainloh-probes", min=0, max=250000)] = 300,
|
||
min_gainloh_size: Annotated[int, typer.Option("--min-gainloh-size", help="kb")] = 500,
|
||
min_mdel_probes: Annotated[int, typer.Option("--min-mdel-probes", min=0, max=250000)] = 10,
|
||
min_mdel_size: Annotated[int, typer.Option("--min-mdel-size", help="kb")] = 25,
|
||
min_mdup_probes: Annotated[int, typer.Option("--min-mdup-probes", min=0, max=250000)] = 10,
|
||
min_mdup_size: Annotated[int, typer.Option("--min-mdup-size", help="kb")] = 50,
|
||
min_mloh_probes: Annotated[int, typer.Option("--min-mloh-probes", min=0, max=250000)] = 500,
|
||
min_mloh_size: Annotated[int, typer.Option("--min-mloh-size", help="kb")] = 3000,
|
||
smoothing: Annotated[int, typer.Option("--smoothing", min=0, max=1000)] = 5,
|
||
max_mosaic_fraction: Annotated[float, typer.Option("--max-mosaic-fraction")] = 1.0,
|
||
min_mosaic_fraction: Annotated[float, typer.Option("--min-mosaic-fraction")] = 0.10,
|
||
filter_qual: Annotated[int, typer.Option("--filter-qual", min=0, max=60)] = 10,
|
||
cn_cap: Annotated[int, typer.Option("--cn-cap", min=3, max=8)] = 4,
|
||
gc_correct: Annotated[bool, typer.Option("--gc-correct/--no-gc-correct")] = True,
|
||
unknown_sex_policy: Annotated[str, typer.Option("--unknown-sex-policy")] = "skip-sex-chromosomes",
|
||
gt_mode: Annotated[str, typer.Option("--gt-mode", help="vcf|compat")] = "vcf",
|
||
svlen_mode: Annotated[str, typer.Option("--svlen-mode", help="signed|positive")] = "signed",
|
||
mask_bed: Annotated[Path | None, typer.Option("--mask-bed", help="Mask BED (default: built-in).")] = None,
|
||
no_bgzip: Annotated[bool, typer.Option("--no-bgzip", help="Plain VCF (cn annotate then refuses it).")] = False,
|
||
mosaic_prior: Annotated[
|
||
float, typer.Option("--mosaic-prior", help="Advanced: mosaic states' switch mass.")
|
||
] = 0.004,
|
||
switch_distance: Annotated[
|
||
float, typer.Option("--switch-distance", help="Advanced: HMM switch distance D (bp).")
|
||
] = 5e8,
|
||
probe_lrr_sd: Annotated[
|
||
bool, typer.Option("--probe-lrr-sd/--no-probe-lrr-sd", help="Advanced: per-probe LRR noise from the model.")
|
||
] = True,
|
||
threads: ThreadsOpt = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""GTCs -> per-sample CNV/LOH/mosaic VCF, segments and summary (CLI-028)."""
|
||
from simurg.cn.events import Thresholds
|
||
from simurg.pipeline.cn import CnCallOptions, run_cn_call
|
||
|
||
if unknown_sex_policy not in ("skip-sex-chromosomes", "female", "male", "error"):
|
||
raise typer.BadParameter("--unknown-sex-policy: skip-sex-chromosomes|female|male|error")
|
||
if gt_mode not in ("vcf", "compat") or svlen_mode not in ("signed", "positive"):
|
||
raise typer.BadParameter("--gt-mode vcf|compat, --svlen-mode signed|positive")
|
||
if not 0 < max_mosaic_fraction <= 1 or not 0 <= min_mosaic_fraction < 1:
|
||
raise typer.BadParameter("mosaic fractions: 0 < max <= 1, 0 <= min < 1")
|
||
th = Thresholds(
|
||
probes={"del": min_del_probes, "dup": min_dup_probes, "loh": min_loh_probes, "gainloh": min_gainloh_probes,
|
||
"mdel": min_mdel_probes, "mdup": min_mdup_probes, "mloh": min_mloh_probes},
|
||
size_bp={"del": _kb(min_del_size), "dup": _kb(min_dup_size), "loh": _kb(min_loh_size),
|
||
"gainloh": _kb(min_gainloh_size), "mdel": _kb(min_mdel_size), "mdup": _kb(min_mdup_size),
|
||
"mloh": _kb(min_mloh_size)},
|
||
max_mosaic_fraction=max_mosaic_fraction, min_mosaic_fraction=min_mosaic_fraction, filter_qual=filter_qual,
|
||
) # fmt: skip
|
||
rep = _reporter("cn call", out, log_json, debug, quiet)
|
||
opts = CnCallOptions(
|
||
out=out, bpm=bpm, model=model, gtcs=gtcs, summary=summary, fasta=fasta, thresholds=th, smoothing=smoothing,
|
||
cn_cap=cn_cap, gc_correct=gc_correct, unknown_sex_policy=unknown_sex_policy, gt_mode=gt_mode,
|
||
svlen_mode=svlen_mode, mask_bed=mask_bed, bgzip=not no_bgzip, mosaic_prior=mosaic_prior,
|
||
switch_d=switch_distance, probe_lrr_sd=probe_lrr_sd, threads=threads, force=force,
|
||
argv=sys.argv[1:],
|
||
) # fmt: skip
|
||
run_guarded(rep, lambda: run_cn_call(opts, rep))
|
||
|
||
|
||
@cn_app.command("annotate")
|
||
def cn_annotate_cmd(
|
||
vcfs: Annotated[Path, typer.Option("--vcfs", help="Output folder of cn call (required).")],
|
||
bundle: Annotated[
|
||
Path | None, typer.Option("--annotation-bundle", help="Bundle from scripts/build_annotation_bundle.sh.")
|
||
] = None,
|
||
out: Annotated[Path | None, typer.Option("--out", help="Output folder (default: --vcfs).")] = None,
|
||
min_qual: Annotated[int, typer.Option("--min-qual", min=0, max=60)] = 20,
|
||
size_basis: Annotated[str, typer.Option("--size-basis", help="effective|raw")] = "effective",
|
||
iscn_min_size: Annotated[int, typer.Option("--iscn-min-size", min=0, help="bp")] = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""cn call output -> per-sample JSON report with ISCN 2020 strings, cytobands and genes (CLI-029)."""
|
||
from simurg.pipeline.cn import CnAnnotateOptions, run_cn_annotate
|
||
|
||
if size_basis not in ("effective", "raw"):
|
||
raise typer.BadParameter("--size-basis effective|raw")
|
||
rep = _reporter("cn annotate", out or vcfs, log_json, debug, quiet)
|
||
opts = CnAnnotateOptions(vcfs=vcfs, bundle=bundle, out=out, min_qual=min_qual, size_basis=size_basis,
|
||
iscn_min_size=iscn_min_size, force=force) # fmt: skip
|
||
run_guarded(rep, lambda: run_cn_annotate(opts, rep))
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
pgx_app = typer.Typer(help="Pharmacogenomics: targeted copy number, star alleles, phenotypes (v2).")
|
||
app.add_typer(pgx_app, name="pgx")
|
||
pgx_cn_app = typer.Typer(help="Targeted PGx copy number (PCN-001).")
|
||
pgx_app.add_typer(pgx_cn_app, name="copy-number")
|
||
|
||
|
||
pgx_db_app = typer.Typer(help="PGx allele-definition database (PGX-001).")
|
||
pgx_app.add_typer(pgx_db_app, name="db")
|
||
|
||
|
||
@pgx_db_app.command("build")
|
||
def pgx_db_build_cmd(
|
||
sources: Annotated[Path, typer.Option("--sources", help="Folder from scripts/pgx/fetch_sources.sh (required).")],
|
||
fasta: Annotated[Path, typer.Option("--fasta", help="GRCh38 FASTA for variant normalisation (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder for pgx_db-<schema>-<sha12>.sqlite.")] = Path("."),
|
||
manifests: Annotated[
|
||
list[Path] | None, typer.Option("--manifests", help="BPM manifest(s) for the probe map (repeatable).")
|
||
] = None,
|
||
csv: Annotated[list[Path] | None, typer.Option("--csv", help="CSV manifest(s), same order (indels).")] = None,
|
||
mask: Annotated[list[Path] | None, typer.Option("--mask", help="Probe mask TSV(s) (PGX-010).")] = None,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Build the PGx DB from public sources (CLI-035, PGX-002)."""
|
||
from simurg.pipeline.pgx_db import PgxDbOptions, run_db_build
|
||
|
||
rep = _reporter("pgx db build", out, log_json, debug, quiet)
|
||
o = PgxDbOptions(sources=sources, fasta=fasta, out=out, manifests=manifests or [], csvs=csv or [],
|
||
masks=mask or [], force=force) # fmt: skip
|
||
run_guarded(rep, lambda: run_db_build(o, rep))
|
||
|
||
|
||
pgx_star_app = typer.Typer(help="Star-allele calling (PGX-011).")
|
||
pgx_app.add_typer(pgx_star_app, name="star-allele")
|
||
|
||
|
||
@pgx_star_app.command("call")
|
||
def pgx_star_call_cmd(
|
||
vcfs: Annotated[list[Path], typer.Option("--vcfs", help="Folder(s) with SNV + PGx CN VCFs (repeatable).")],
|
||
db: Annotated[Path, typer.Option("--db", help="PGx DB from pgx db build (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
population: Annotated[str, typer.Option("--population", help="Frequency prior population.")] = "global",
|
||
max_candidates: Annotated[int, typer.Option("--max-candidates", min=1)] = 10,
|
||
feasible_ratio: Annotated[float, typer.Option("--feasible-ratio", min=0.0, max=1.0)] = 0.5,
|
||
low_gs: Annotated[float, typer.Option("--low-gs")] = 0.15,
|
||
no_cn: Annotated[bool, typer.Option("--no-cn", help="Run without PGx CN VCFs.")] = False,
|
||
allow_foreign_vcf: Annotated[bool, typer.Option("--allow-foreign-vcf")] = False,
|
||
gene: Annotated[list[str] | None, typer.Option("--gene", help="Restrict to gene(s).")] = None,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Call star-allele diplotypes (CLI-033, PGX-012 ... PGX-021)."""
|
||
from simurg.pipeline.pgx_star import StarOptions, run_star_call
|
||
|
||
rep = _reporter("pgx star-allele call", out, log_json, debug, quiet)
|
||
o = StarOptions(vcfs=vcfs, db=db, out=out, population=population, max_candidates=max_candidates,
|
||
feasible_ratio=feasible_ratio, low_gs=low_gs, no_cn=no_cn, allow_foreign_vcf=allow_foreign_vcf,
|
||
genes=gene or [], force=force) # fmt: skip
|
||
run_guarded(rep, lambda: run_star_call(o, rep))
|
||
|
||
|
||
def _pgx_phenotype(calls: Path, db: Path, out: Path | None, guideline: str, force: bool, log_json: bool,
|
||
debug: bool, quiet: bool, name: str) -> None: # fmt: skip
|
||
from simurg.pipeline.pgx_pheno import PhenoOptions, run_phenotype
|
||
|
||
if guideline not in ("cpic", "dpwg"):
|
||
raise typer.BadParameter("--guideline cpic|dpwg")
|
||
rep = _reporter(name, out or calls, log_json, debug, quiet)
|
||
o = PhenoOptions(calls=calls, db=db, out=out, guideline=guideline, force=force)
|
||
run_guarded(rep, lambda: run_phenotype(o, rep))
|
||
|
||
|
||
@pgx_app.command("phenotype")
|
||
def pgx_phenotype_cmd(
|
||
calls: Annotated[Path, typer.Option("--calls", help="Output folder of pgx star-allele call (required).")],
|
||
db: Annotated[Path, typer.Option("--db", help="PGx DB (required).")],
|
||
out: Annotated[Path | None, typer.Option("--out", help="Output folder (default: --calls).")] = None,
|
||
guideline: Annotated[str, typer.Option("--guideline", help="cpic|dpwg")] = "cpic",
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Phenotypes from star-allele calls: per-sample PGx JSON and diplotype_annotations.tsv (CLI-034)."""
|
||
_pgx_phenotype(calls, db, out, guideline, force, log_json, debug, quiet, "pgx phenotype")
|
||
|
||
|
||
@pgx_app.command("annotate", hidden=True)
|
||
def pgx_annotate_cmd(
|
||
calls: Annotated[Path, typer.Option("--calls")],
|
||
db: Annotated[Path, typer.Option("--db")],
|
||
out: Annotated[Path | None, typer.Option("--out")] = None,
|
||
guideline: Annotated[str, typer.Option("--guideline")] = "cpic",
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Alias of pgx phenotype (CLI-034)."""
|
||
_pgx_phenotype(calls, db, out, guideline, force, log_json, debug, quiet, "pgx annotate")
|
||
|
||
|
||
@pgx_cn_app.command("train")
|
||
def pgx_cn_train_cmd(
|
||
gtcs: Annotated[Path, typer.Option("--gtcs", help="Folder searched recursively for training GTCs (required).")],
|
||
bpm: Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")],
|
||
fasta: Annotated[Path, typer.Option("--fasta", help="GRCh38 FASTA (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder.")] = Path("."),
|
||
mask: Annotated[Path | None, typer.Option("--mask", help="Probe mask TSV (IN-052).")] = None,
|
||
regions: Annotated[Path | None, typer.Option("--regions", help="Region BED (default: built-in v1).")] = None,
|
||
summary: Annotated[Path | None, typer.Option("--summary", help="sample_summary.tsv (assay control).")] = None,
|
||
platform: Annotated[str, typer.Option("--platform", help="auto|LCG|EX")] = "auto",
|
||
min_samples: Annotated[int, typer.Option("--min-samples", min=1)] = 96,
|
||
min_passing: Annotated[int, typer.Option("--min-passing", min=1)] = 90,
|
||
max_samples: Annotated[int, typer.Option("--max-samples", min=1)] = 3000,
|
||
train_lrr_sd_max: Annotated[float, typer.Option("--train-lrr-sd-max")] = 0.20,
|
||
train_call_rate_min: Annotated[float, typer.Option("--train-call-rate-min")] = 0.99,
|
||
train_assay_control_min: Annotated[float, typer.Option("--train-assay-control-min")] = 1.0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Train a PGx CN model (CLI-032, PCN-005)."""
|
||
from simurg.pipeline.pgx_cn import PgxTrainOptions, run_train
|
||
|
||
if platform not in ("auto", "LCG", "EX"):
|
||
raise typer.BadParameter("--platform auto|LCG|EX")
|
||
rep = _reporter("pgx copy-number train", out, log_json, debug, quiet)
|
||
o = PgxTrainOptions(gtcs=gtcs, bpm=bpm, fasta=fasta, out=out, mask=mask, regions=regions, summary=summary,
|
||
platform=platform, min_samples=min_samples, min_passing=min_passing, max_samples=max_samples,
|
||
lrr_sd_max=train_lrr_sd_max, call_rate_min=train_call_rate_min,
|
||
assay_control_min=train_assay_control_min, force=force) # fmt: skip
|
||
run_guarded(rep, lambda: run_train(o, rep))
|
||
|
||
|
||
@pgx_cn_app.command("call")
|
||
def pgx_cn_call_cmd(
|
||
gtcs: Annotated[Path, typer.Option("--gtcs", help="Folder searched recursively for GTC files (required).")],
|
||
model: Annotated[Path, typer.Option("--model", help="PGx CN model (.simurg-pgxcn, required).")],
|
||
fasta: Annotated[Path, typer.Option("--fasta", help="GRCh38 FASTA with alt contigs (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
min_batch: Annotated[int, typer.Option("--min-batch", min=1)] = 24,
|
||
min_passing: Annotated[int, typer.Option("--min-passing", min=1)] = 22,
|
||
recommended_passing: Annotated[int, typer.Option("--recommended-passing", min=1)] = 96,
|
||
lrr_sd_max: Annotated[float, typer.Option("--lrr-sd-max")] = 0.20,
|
||
cluster_mismatch: Annotated[str, typer.Option("--cluster-mismatch", help="error|warn")] = "error",
|
||
no_bgzip: Annotated[bool, typer.Option("--no-bgzip")] = False,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Call targeted PGx copy number per region (CLI-031, PCN-009)."""
|
||
from simurg.pipeline.pgx_cn import PgxCallOptions, run_call
|
||
|
||
if cluster_mismatch not in ("error", "warn"):
|
||
raise typer.BadParameter("--cluster-mismatch error|warn")
|
||
if min_passing > min_batch:
|
||
raise typer.BadParameter("--min-passing must be <= --min-batch")
|
||
rep = _reporter("pgx copy-number call", out, log_json, debug, quiet)
|
||
o = PgxCallOptions(gtcs=gtcs, model=model, fasta=fasta, out=out, min_batch=min_batch, min_passing=min_passing,
|
||
recommended_passing=recommended_passing, lrr_sd_max=lrr_sd_max,
|
||
cluster_mismatch=cluster_mismatch, bgzip=not no_bgzip, force=force) # fmt: skip
|
||
run_guarded(rep, lambda: run_call(o, rep))
|
||
|
||
|
||
# --------------------------------------------------------------------------------------------------------
|
||
methyl_app = typer.Typer(help="Methylation arrays: control QC, detection, beta values (MET-001).")
|
||
app.add_typer(methyl_app, name="methyl")
|
||
|
||
|
||
@methyl_app.command("qc")
|
||
def methyl_qc_cmd(
|
||
manifest: Annotated[Path, typer.Option("--manifest", help="Methylation CSV manifest (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
idats: Annotated[Path | None, typer.Option("--idats", help="Folder searched recursively for IDAT pairs.")] = None,
|
||
sheet: Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")] = None,
|
||
config: Annotated[Path | None, typer.Option("--config", help="QC threshold YAML.")] = None,
|
||
profile: Annotated[str, typer.Option("--profile", help="QC profile name, 'auto' or 'none'.")] = "auto",
|
||
threshold: Annotated[list[str] | None, typer.Option("--threshold", help="KEY=VALUE (repeatable).")] = None,
|
||
bg_offset: Annotated[float | None, typer.Option("--bg-offset", help="Background offset x (3000).")] = None,
|
||
bs_set: Annotated[str, typer.Option("--bs-conv-red-bg-set", help="U3-U5 (default) or U4-U6.")] = "U3-U5",
|
||
pval: Annotated[float, typer.Option("--pval-threshold", help="Detection p threshold.")] = 0.05,
|
||
beta_offset: Annotated[float, typer.Option("--beta-offset", help="alpha in M/(M+U+alpha).")] = 0.0,
|
||
dye_bias: Annotated[str, typer.Option("--dye-bias", help="linear|nonlinear")] = "linear",
|
||
min_prop: Annotated[
|
||
float | None, typer.Option("--min-prop-passing", help="Fail samples below this detection rate.")
|
||
] = None,
|
||
pca_full: Annotated[int, typer.Option("--pca-max-samples-full", min=2)] = 100,
|
||
pca_probes: Annotated[int, typer.Option("--pca-probes", min=2)] = 10_000,
|
||
threads: Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")] = 0,
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Methylation control QC: 21 control metrics, detection p-values, noob/dye-bias corrected β (MET-001)."""
|
||
from simurg.pipeline.methyl import MethylQcOptions, run_methyl_qc
|
||
|
||
if dye_bias not in ("linear", "nonlinear"):
|
||
raise typer.BadParameter("--dye-bias must be linear or nonlinear")
|
||
if bs_set not in ("U3-U5", "U4-U6"):
|
||
raise typer.BadParameter("--bs-conv-red-bg-set must be U3-U5 or U4-U6")
|
||
if not 0 < pval <= 1:
|
||
raise typer.BadParameter("--pval-threshold must be in (0, 1]")
|
||
rep = _reporter("methyl qc", out, log_json, debug, quiet)
|
||
opts = MethylQcOptions(
|
||
out=out, manifest=manifest, idats=idats, sheet=sheet, config=config,
|
||
profile=None if profile == "none" else profile, thresholds=threshold or [], bg_offset=bg_offset,
|
||
bs_conv_red_bg_set=bs_set, pval_threshold=pval, beta_offset=beta_offset, dye_bias=dye_bias,
|
||
min_prop_passing=min_prop, pca_max_samples_full=pca_full, pca_probes=pca_probes, threads=threads,
|
||
force=force, argv=sys.argv[1:],
|
||
) # fmt: skip
|
||
run_guarded(rep, lambda: run_methyl_qc(opts, rep))
|
||
|
||
|
||
@methyl_app.command("convert-manifest")
|
||
def methyl_convert_cmd(
|
||
manifest: Annotated[Path, typer.Option("--manifest", help="Methylation CSV manifest (required).")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder (required).")],
|
||
force: Annotated[bool, typer.Option("--force", help="Replace existing outputs.")] = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Methylation manifest -> probes.tsv and controls.tsv (MET-003)."""
|
||
from simurg.pipeline.methyl import run_convert_manifest
|
||
|
||
rep = _reporter("methyl convert-manifest", out, log_json, debug, quiet)
|
||
run_guarded(rep, lambda: run_convert_manifest(manifest, out, force, rep))
|
||
|
||
|
||
def _qc_cohort(
|
||
command: str,
|
||
out: Path,
|
||
bpm: Path,
|
||
gtcs: Path | None,
|
||
sheet: Path | None,
|
||
egt: Path | None,
|
||
threads: int,
|
||
force: bool,
|
||
log_json: bool,
|
||
debug: bool,
|
||
quiet: bool,
|
||
*,
|
||
all_pairs: bool = False,
|
||
pedigree_from_sheet: bool = True,
|
||
median: str = "reference",
|
||
config: Path | None = None,
|
||
profile: str | None = None,
|
||
threshold_call_rate: float | None = None,
|
||
threshold_lrr_sd: float | None = None,
|
||
threshold: list[str] | None = None,
|
||
) -> None:
|
||
from simurg.pipeline.qc import QcCohortOptions, run_qc_cohort
|
||
from simurg.qc.thresholds import parse_cli_pairs, resolve_thresholds
|
||
|
||
if median not in ("reference", "exact"):
|
||
raise typer.BadParameter("--median must be reference or exact")
|
||
rep = _reporter(f"qc {command}", out, log_json, debug, quiet)
|
||
|
||
def body() -> int:
|
||
cli: dict[str, Any] = parse_cli_pairs(list(threshold or []))
|
||
if threshold_call_rate is not None:
|
||
cli["callRate"] = threshold_call_rate
|
||
if threshold_lrr_sd is not None:
|
||
cli["logRDev"] = threshold_lrr_sd
|
||
th = resolve_thresholds(config=config, profile=profile, cli=cli)
|
||
opts = QcCohortOptions(
|
||
command=command,
|
||
out=out,
|
||
bpm=bpm,
|
||
gtcs=gtcs,
|
||
sheet=sheet,
|
||
egt=egt,
|
||
threads=threads,
|
||
force=force,
|
||
all_pairs=all_pairs,
|
||
pedigree_from_sheet=pedigree_from_sheet,
|
||
median=median,
|
||
thresholds=th,
|
||
argv=sys.argv[1:],
|
||
)
|
||
return run_qc_cohort(opts, rep)
|
||
|
||
run_guarded(rep, body)
|
||
|
||
|
||
GtcsOpt = Annotated[Path | None, typer.Option("--gtcs", help="Folder searched recursively for GTC files.")]
|
||
SheetOpt = Annotated[Path | None, typer.Option("--sheet", help="Sample sheet (CSV).")]
|
||
BpmOpt = Annotated[Path, typer.Option("--bpm", help="BPM manifest (required).")]
|
||
OutOpt = Annotated[Path, typer.Option("--out", help="Output folder (required).")]
|
||
ThreadsOpt = Annotated[int, typer.Option("--threads", min=0, max=1024, help="Worker processes (0 = auto).")]
|
||
ForceOpt = Annotated[bool, typer.Option("--force", help="Replace existing outputs.")]
|
||
MedianOpt = Annotated[str, typer.Option("--median", help="reference (default, bit-compatible) or exact.")]
|
||
|
||
|
||
@qc_app.command("samples")
|
||
def qc_samples_cmd(
|
||
out: OutOpt,
|
||
bpm: BpmOpt,
|
||
gtcs: GtcsOpt = None,
|
||
sheet: SheetOpt = None,
|
||
config: Annotated[Path | None, typer.Option("--config", help="QC threshold YAML.")] = None,
|
||
profile: Annotated[str | None, typer.Option("--profile", help="QC profile name or 'auto'.")] = None,
|
||
threshold_call_rate: Annotated[float | None, typer.Option("--threshold-call-rate")] = None,
|
||
threshold_lrr_sd: Annotated[float | None, typer.Option("--threshold-lrr-sd")] = None,
|
||
threshold: Annotated[list[str] | None, typer.Option("--threshold", help="KEY=VALUE (repeatable).")] = None,
|
||
all_pairs: Annotated[bool, typer.Option("--all-pairs", help="Write every sample pair.")] = False,
|
||
median: MedianOpt = "reference",
|
||
threads: ThreadsOpt = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Sample QC from GTCs: het rate, contamination, relatedness, flags (sample_qc.tsv)."""
|
||
_qc_cohort(
|
||
"samples",
|
||
out,
|
||
bpm,
|
||
gtcs,
|
||
sheet,
|
||
None,
|
||
threads,
|
||
force,
|
||
log_json,
|
||
debug,
|
||
quiet,
|
||
all_pairs=all_pairs,
|
||
median=median,
|
||
config=config,
|
||
profile=profile,
|
||
threshold_call_rate=threshold_call_rate,
|
||
threshold_lrr_sd=threshold_lrr_sd,
|
||
threshold=threshold,
|
||
)
|
||
|
||
|
||
@qc_app.command("contamination")
|
||
def qc_contamination_cmd(
|
||
out: OutOpt,
|
||
bpm: BpmOpt,
|
||
gtcs: GtcsOpt = None,
|
||
sheet: SheetOpt = None,
|
||
median: MedianOpt = "reference",
|
||
threads: ThreadsOpt = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""BAF-regression contamination estimate per sample (contamination.tsv)."""
|
||
_qc_cohort("contamination", out, bpm, gtcs, sheet, None, threads, force, log_json, debug, quiet, median=median)
|
||
|
||
|
||
@qc_app.command("relatedness")
|
||
def qc_relatedness_cmd(
|
||
out: OutOpt,
|
||
bpm: BpmOpt,
|
||
gtcs: GtcsOpt = None,
|
||
sheet: SheetOpt = None,
|
||
pedigree_from_sheet: Annotated[
|
||
bool, typer.Option("--pedigree-from-sheet/--no-pedigree", help="Use Parent1/Parent2/Replicate.")
|
||
] = True,
|
||
all_pairs: Annotated[bool, typer.Option("--all-pairs", help="Write every sample pair.")] = False,
|
||
threads: ThreadsOpt = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""KING-robust kinship, duplicates and Mendelian errors (relatedness.tsv, mendel.tsv)."""
|
||
_qc_cohort(
|
||
"relatedness",
|
||
out,
|
||
bpm,
|
||
gtcs,
|
||
sheet,
|
||
None,
|
||
threads,
|
||
force,
|
||
log_json,
|
||
debug,
|
||
quiet,
|
||
all_pairs=all_pairs,
|
||
pedigree_from_sheet=pedigree_from_sheet,
|
||
)
|
||
|
||
|
||
@qc_app.command("loci")
|
||
def qc_loci_cmd(
|
||
out: OutOpt,
|
||
bpm: BpmOpt,
|
||
egt: Annotated[Path, typer.Option("--egt", help="EGT cluster file (required).")],
|
||
gtcs: GtcsOpt = None,
|
||
sheet: SheetOpt = None,
|
||
threads: ThreadsOpt = 0,
|
||
force: ForceOpt = False,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Per-locus summary statistics across samples (locus_summary.tsv, OUT-072)."""
|
||
_qc_cohort("loci", out, bpm, gtcs, sheet, egt, threads, force, log_json, debug, quiet)
|
||
|
||
|
||
@qc_app.command("report")
|
||
def qc_report_cmd(
|
||
data: Annotated[list[str], typer.Option("--data", help="Dataset folder(s): one, a comma list, or a parent.")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output folder.")] = Path("."),
|
||
label: Annotated[
|
||
list[str] | None, typer.Option("--label", help="Dataset labels (list or discovery order).")
|
||
] = None,
|
||
config: Annotated[Path | None, typer.Option("--config", help="QC threshold YAML (QC-020).")] = None,
|
||
profile: Annotated[str | None, typer.Option("--profile", help="QC profile name or 'auto'.")] = None,
|
||
threshold_call_rate: Annotated[float | None, typer.Option("--threshold-call-rate")] = None,
|
||
threshold_lrr_sd: Annotated[float | None, typer.Option("--threshold-lrr-sd")] = None,
|
||
threshold: Annotated[list[str] | None, typer.Option("--threshold", help="KEY=VALUE (repeatable).")] = None,
|
||
output_format: Annotated[str, typer.Option("--output-format", help="xlsx|csv|both")] = "xlsx",
|
||
report_name: Annotated[str | None, typer.Option("--report-name", help="Fixed output file stem.")] = None,
|
||
drop_metadata: Annotated[
|
||
list[str] | None, typer.Option("--drop-metadata", help="Metadata columns to drop.")
|
||
] = None,
|
||
keep_metadata: Annotated[list[str] | None, typer.Option("--keep-metadata", help="Metadata allowlist.")] = None,
|
||
no_metadata: Annotated[bool, typer.Option("--no-metadata", help="Embed no metadata columns.")] = False,
|
||
redact_paths: Annotated[bool, typer.Option("--redact-paths", help="Blank sample_folder.")] = False,
|
||
split_by: Annotated[str, typer.Option("--split-by", help="none|dataset")] = "none",
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Self-contained HTML QC report and QC table from dataset folders (QC-040 … QC-063)."""
|
||
from simurg.pipeline.qc_report import QcReportOptions, run_qc_report
|
||
from simurg.qc.thresholds import parse_cli_pairs, resolve_thresholds
|
||
|
||
rep = _reporter("qc report", None, log_json, debug, quiet)
|
||
|
||
def body() -> int:
|
||
cli: dict[str, Any] = parse_cli_pairs(list(threshold or []))
|
||
if threshold_call_rate is not None:
|
||
cli["callRate"] = threshold_call_rate
|
||
if threshold_lrr_sd is not None:
|
||
cli["logRDev"] = threshold_lrr_sd
|
||
th = resolve_thresholds(config=config, profile=profile, cli=cli)
|
||
opts = QcReportOptions(
|
||
data=list(data),
|
||
out=out,
|
||
labels=list(label) if label else None,
|
||
output_format=output_format,
|
||
report_name=report_name,
|
||
drop_metadata=list(drop_metadata or []),
|
||
keep_metadata=list(keep_metadata) if keep_metadata else None,
|
||
no_metadata=no_metadata,
|
||
redact_paths=redact_paths,
|
||
split_by=split_by,
|
||
thresholds=th,
|
||
)
|
||
return run_qc_report(opts, rep)
|
||
|
||
run_guarded(rep, body)
|
||
|
||
|
||
@qc_app.command("suggest-thresholds")
|
||
def qc_suggest_cmd(
|
||
data: Annotated[list[Path], typer.Option("--data", help="Dataset folder(s) with controls_qc.tsv.")],
|
||
out: Annotated[Path, typer.Option("--out", help="Output YAML file (required).")],
|
||
k: Annotated[float, typer.Option("--k", min=0.0, help="Spread multiplier.")] = 4.0,
|
||
min_samples: Annotated[int, typer.Option("--min-samples", min=1, help="Minimum samples per metric.")] = 30,
|
||
config: Annotated[Path | None, typer.Option("--config", help="Current thresholds (YAML).")] = None,
|
||
profile: Annotated[str | None, typer.Option("--profile", help="Current profile.")] = None,
|
||
log_json: LogJson = False,
|
||
debug: Debug = False,
|
||
quiet: Quiet = False,
|
||
) -> None:
|
||
"""Data-driven QC threshold suggestion (QC-026)."""
|
||
from simurg.pipeline.qc import run_suggest_thresholds
|
||
from simurg.qc.thresholds import resolve_thresholds
|
||
|
||
rep = _reporter("qc suggest-thresholds", None, log_json, debug, quiet)
|
||
folders = [Path(p) for d in data for p in str(d).split(",") if p]
|
||
|
||
def body() -> int:
|
||
th = resolve_thresholds(config=config, profile=profile)
|
||
return run_suggest_thresholds(folders, out, th, rep, k=k, min_samples=min_samples)
|
||
|
||
run_guarded(rep, body)
|
||
|
||
|
||
if __name__ == "__main__": # pragma: no cover
|
||
app()
|