"""BCF 2.2 writer and CSI index (spec OUT-040, OUT-038). Records are built from typed values; the file is BGZF-compressed and indexed with a CSI index (min_shift 14, depth 5, the pseudo-bin carries record counts). [DOC: VCF/BCF specification v4.3 ยง6 (BCF2), CSI index format โ€” layouts only] """ from __future__ import annotations import struct from collections.abc import Sequence from simurg.formats.bgzf import PSEUDO_BIN, BgzfWriter, bgzf_compress, reg2bin INT8, INT16, INT32, FLOAT, CHAR = 1, 2, 3, 5, 7 FLOAT_MISSING = struct.pack(" bytes: if n < 15: return bytes([(n << 4) | t]) return bytes([(15 << 4) | t]) + typed_ints([n]) def typed_ints(values: Sequence[int]) -> bytes: """Smallest integer type that holds every value (missing/vector-end sentinels excluded from the range).""" lo, hi = (min(values), max(values)) if values else (0, 0) if lo >= -120 and hi <= 127: return _type_byte(len(values), INT8) + struct.pack(f"<{len(values)}b", *values) if lo >= -32760 and hi <= 32767: return _type_byte(len(values), INT16) + struct.pack(f"<{len(values)}h", *values) return _type_byte(len(values), INT32) + struct.pack(f"<{len(values)}i", *values) def typed_string(s: str) -> bytes: b = s.encode() return _type_byte(len(b), CHAR) + b def float_bytes(values: Sequence[float | None]) -> bytes: out = bytearray() for v in values: out += FLOAT_MISSING if v is None or v != v else struct.pack(" tuple[int, bytes]: """FORMAT/GT for all samples: width = max ploidy; unphased (a+1)<<1; missing 0; haploid padded with END.""" width = max(1, max(ploidies)) vals: list[int] = [] for g, p in zip(genotypes, ploidies, strict=True): p = max(p, 1) cells = [0] * p if g is None else [(a + 1) << 1 for a in g] cells += [INT8_VECTOR_END] * (width - len(cells)) vals += cells return width, bytes(vals_to_int8(vals)) def vals_to_int8(vals: Sequence[int]) -> bytes: return struct.pack(f"<{len(vals)}b", *vals) def encode_record( contig_id: int, pos0: int, rlen: int, vid: str, alleles: Sequence[str], filter_id: int, n_sample: int, fmt: Sequence[tuple[int, bytes, bytes]], info: Sequence[tuple[int, bytes]] = (), ) -> bytes: """``fmt``: (format dictionary index, type-descriptor bytes, per-sample payload) in FORMAT order; ``info``: (dictionary index, typed value) pairs.""" shared = bytearray() shared += struct.pack(" None: self.refs: list[dict[str, object]] = [{} for _ in range(n_ref)] def add(self, rid: int, beg: int, end: int, vstart: int, vend: int) -> None: r = self.refs[rid] if not r: r.update(bins={}, n=0, first=vstart, last=vend) bins: dict[int, list[list[int]]] = r["bins"] # type: ignore[assignment] b = reg2bin(beg, end) chunks = bins.setdefault(b, []) if chunks and chunks[-1][1] == vstart: chunks[-1][1] = vend else: chunks.append([vstart, vend]) r["n"] = int(r["n"]) + 1 # type: ignore[call-overload] r["last"] = vend def encode(self) -> bytes: out = bytearray(b"CSI\x01") + struct.pack(" None: self.w = BgzfWriter(fh) # type: ignore[arg-type] text = header_text.encode() + b"\0" self.w.write_record(b"BCF\x02\x02" + struct.pack(" tuple[int, int]: return self.w.write_record(record) def close(self) -> None: self.w.close() def header_dictionaries(header_text: str) -> tuple[dict[str, int], dict[str, int]]: """(string dictionary: FILTER/INFO/FORMAT IDs โ†’ index, contig dictionary: name โ†’ index) as htslib builds them.""" strings: dict[str, int] = {"PASS": 0} contigs: dict[str, int] = {} for line in header_text.splitlines(): for kind in ("FILTER", "INFO", "FORMAT"): if line.startswith(f"##{kind}=", 1)[0] strings.setdefault(key, len(strings)) if line.startswith("##contig=", 1)[0] contigs.setdefault(name, len(contigs)) return strings, contigs def typed_floats(values: Sequence[float]) -> bytes: return _type_byte(len(values), FLOAT) + float_bytes(values)